The singer, 22, who sustained the injury while playing basketball, posted a picture of his swollen left foot on Instagram.
"Gonna be taking it easy tonight on stage sprained my ankle playing ball smh all good still gonna crush," he wrote.
Bieber also shared a second image of his hurt foot - as well as a video clip showing off some one-legged dance moves from his night's performance, which he powered through.
The "Baby" hitmaker's next tour stop is in Indianapolis.