The movie includes big names likes Ian McKellen, Danny DeVito, Sylvester Stallone, Patrick Warburton, Gilbert Gottfried, Raven-Symone and Harvey Fierstei, reported Aceshowbiz.
While Cuoco, 29, will voice the character of Zoe Huntington, Krasinski, 35, will lend his vocals to Owen.
"I can't tell you how excited I am to see John and Kaley portray Owen and Zoe Huntington in 'Animal Crackers'.
"We just did our first session with John this week and what an amazing experience to hear someone as incredibly talented as John take a character from my imagination and bring him to life with such humour and soul," the film's director and co-writer Scott Christian Sava said.