The 38-year-old rapper stole the microphone off of the best man during the toast at the wedding, which was held at Wynwood Walls in Miami on Saturday, to repeat the moment he interjected Taylor Swift at the MTV Music Awards in 2009.
Speaking at the marital ceremony, the 'Real Friends' musician said: "Imma let you finish."
However, Kanye's joke was accepted well by wedding guests, which include Ryan Seacrest, Hulk Hogan and Kourtney Kardashian.