The 44-year-old director posted the photo on Twitter in which his mother Hiroo Johar is seen cuddling the babies.
"Loves of my life!!!! My mom and my babies #roohiandyash," Karan captioned the photo.
The filmmaker welcomed - a baby boy and girl- who were born through surrogacy, in February.
Karan named his son Yash, after his late father Yash Johar.