Karzai, whose relations with Washington have steadily deteriorated over the years, has compiled a list of dozens of attacks that he believes the US government may have been involved in, the Washington Post wrote, citing unnamed Afghan officials.
Karzai even harbors suspicions that the Americans may have been behind an attack this month on a Lebanese restaurant frequented by foreigners in Kabul, the newspaper said, quoting a presidential palace official.
The Taliban often claims responsibility for bombings employing homemade explosives and other assaults, including the recent deadly attack on the Lebanese restaurant.
In Kabul, Afghan officials were unavailable to comment on the report. US officials privately scoffed at the allegations.
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The United States paved the way for Karzai's coming to power by toppling the Taliban regime in 2001 over its support of Al-Qaeda.
Washington and its NATO allies have since poured billions of dollars of aid money into Afghanistan and sent tens of thousands of troops to the country to battle the Taliban.
The Afghan leader has long railed against US and NATO military operations that have left Afghan civilians dead but it remains unclear why the mercurial president would try to pin blame on the Americans for attacks associated with the insurgents.
Karzai has held up a crucial bilateral security agreement negotiated with the US government that would allow for a smaller contingent of American troops to stay in the country after the end of the year.
NATO combat troops are due to withdraw by December and without a legal agreement, the United States and NATO would have to drop the idea of a post-2014 force.
It also was possible Karzai wanted to demonstrate he was no puppet of Washington or that he wanted to persuade the Taliban he was ready for reconciliation talks.