The 54-year-old comedian took to Twitter and accused the former Disney star of lip-syncing her new single "Cool for the Summer" at the event, reported Aceshowbiz.
"Demis live singing was very obviously live if you know I'm saying. And then the rest of the track or tracks. #atleastshesang-ish," she wrote.
Lovato, 23, eventually found the tweet and responded to the allegation by insinuating that the former "Fashion Police" host was obsessed with her.
"Happy to see you're still stanning me @kathygriffin. Dont worry, my new album is out October 16. But you probably already knew that," she said.
Griffin and Lovato had a spat last year after the comedienne slammed the "Heart Attack" hitmaker.