The 28-year-old -- who ran an astonishing time of 1min 40.91sec to take gold in 2012 -- took to Twitter to reveal he could not take his place in the London Stadium for the championships.
"It's sad and disappointing to announce my no show in the forthcoming #london2017 WC due to a quad muscle strain," tweeted Rudisha, who was the defending champion.
The absence of the two-time Olympic champion -- whose run in 2012 was acclaimed as the standout performance of the Games by London 2012 chairman and athletics great Sebastian Coe -- could pave the way for compatriot Emmanuel Korir or the man who chased him home in London in 2012, Nijel Amos of Botswana.