Uthappa made 87 off 47 balls while Gambhir scored 62 off 46 balls as the duo stitched 158 runs off just 85 balls for the second wicket to power KKR to the comprehensive victory.
Chasing 183 for win, KKR rode on Uthappa and Gambhir's brilliant knocks to overwhelm the target in 18.1 overs.
Earlier, Steven Smith led from the front with an unbeaten 51 to lift Rising Pune Supergiant to a challenging 182 for five.
Besides Smith, Ajinkya Rahane (46 off 41 balls), Rahul Tripathi (38 off 23), Mahendra Singh Dhoni (23 off 11) and Daniel Christian (16 off 6) also made useful contributions for the home team.
Left-arm chinaman bowler Kuldeep Yadav was the pick of the bowlers for KKR with figures of two for 31.
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