Pietersen, who was bought by Daredevils for USD 1.5 million (Rs 9 crore), had sustained an injury on his right hand little finger during fielding drills with Surrey at The Oval last month.
Vice-captain Dinesh Karthik will lead the team tomorrow, Daredevils assistant coach Eric Simons said.
"He (Pietersen) did not play in the practice match and he won't be considered for tomorrow's game. Dinesh Karthik will captain the side," Simons said.
Simons said Pietersen himself was disappointed on missing out since he has been looking forward to playing for a long time now.
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"It is tough for KP himself. He is someone who is very passionate. Even though he has not been able to take much part in practice sessions, he has been there for all of them. He is enthusiastic about his new role for Daredevils this year and it's disappointing for him as much as it's for us," he said.
This means that Pietersen's post-England career will have to be delayed. He was sacked in the wake of England's 0-5 Ashes whitewash in Australia last winter, which made him available for a full IPL season for the first time.