The 57-year-old will take over from Emmy-winning television star Jackee Harry for a week in Rick Crom's off-Broadway production, which merges celebrity and political scandals with catchy music, reported New York Post.
"I've wanted to do theatre but on Broadway the shows play for such a long, extended time," Toya said.
Meanwhile, the singer-songwriter has been tied up in the wrongful death lawsuit filed by her family against AEG live.
The Jackson's are suing the company as they believe they negligently hired and supervised Dr Conrad Murray, who is currently serving four years imprisonment for involuntary manslaughter.
Only one member of the Jackson family are allowed to be present in the court room at one time, which Toya says has been challenging for her mother, Katherine Jackson.
"I speak to my mother every day. This has been very taxing on her. But she's so strong. She's holding up so well. We were all there for the last trial," she said.