The fishermen were arrested while they were reportedly fishing off Neduntheevu, Fisheries Inspector Bala said.
They were subsequently taken to Kangesanthurai.
Three boats of the fishermen were also seized, the official added.
On August 8, nearly 50 fishermen from Pudukottai and Jagadapattinam districts were arrested by the Navy of the island nation for allegedly poaching in Lankan waters.
They were subsequently remanded to judicial custody till August 23 by a court in Oorkavalthurai and taken to a jail in Jaffna.