London chief reporter Gavin Sherriff and entertainment features writer Darryl Smith, journalists with Scottish weekly The Sunday Post, are all that remains of the famed institution.
"We knew as journalists that this was an important part of history for our profession, which we love," Smith told AFP.
"There is still that attachment to Fleet Street as a centre of the profession, and we are that final torchbearer."
AFP's departure from Fleet Street in 2009 left the London bureau of Scottish publishers DC Thomson as the last remaining newsroom in the industry's spiritual home, now overtaken by investment banks and legal offices.
DC Thomson will continue to produce non-news publications from 185 and 186 Fleet Street, with its nationally-protected edifice bearing the names of its newspapers.
The buildings, occupied by DC Thomson since the 1880s, certainly have history.
The first authentic English-language versions of The Communist Manifesto were printed at number 185, while Sweeney Todd, "the Demon Barber of Fleet Street", reputedly slit the throats of his customers at number 186.
Sherriff has worked on Fleet Street for 32 years, in a career that has seen him interview notorious killers, give evidence in England's central criminal court, loop the loop in a military jet and fly to Jersey to feed bananas to a gorilla.
"I do remember coming in as a youngster into an office full of (cigarette) smoke. You could barely see across the room, and it was very noisy with people bashing away at typewriters," the 54-year-old told AFP.
Fleet Street began its association with publishing in 1500 when Wynkyn de Worde built London's first printing press next to Saint Bride's, still known as the "journalists' church" and said to have been the inspiration for tiered wedding cakes.
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