Wayne, 32, was performing at Nova Southeastern University in Fort Lauderdale, reported Ace Showbiz.
The rapper wanted to do freestyle over O.T. Genasis' "CoCo" beat but couldn't when the DJ played the original track instead of the instrumental.
The glitch set him off. He reportedly chucked his mic at the clueless DJ and refused to play the rest of the show.
It's been an eventful week for the Wayne. A couple days ago, a SWAT team swamped his home in Miami in response to an alarming call about fatal shootings at the residence.