The 29-year-old singer tweeted Piers Morgan to let him know that she received her "annual" invite for the show, and that "it's still a no" from her.
"Hey @piersmorgan, just got my annual email from one of your life stories producer (sic). It's still a no from me, but thanks again," Allen tweeted.
Morgan then responded to the star on Twitter, saying he knows she will "crack eventually".
"No problem @lilyallen - we both know you'll crack eventually. #LifeStories," Morgan tweeted.
'Piers Morgan's Life Stories' sees famous faces interviewed by Morgan about the highs and lows in their lives.