Set in the past, the film features Karel Roden as as a knight forced to confront his own deepest inner fears when his young son goes missing after running away from home inspired by stories of the Crusades.
It is the first time in 15 years that the festival's famous Crystal Globe for main prize has gone to a Czech film, according to The Hollywood Reporter.
Slovakian filmmaker Peter Bebjak received the best director honour for "The Line", about a cigarette smuggling syndicate that works the Ukrainian border with Slovakia.
Alexander Yatsenko won the best actor award for his role in Boris Khlebnikov's "Arrhythmia".