The half-an-hour 12 song set, filmed at the Washington Coliseum in 1964, was the band's first full US gig, reported BBC online.
It was originally broadcast to two million American cinema-goers in March 1964, a month after it was recorded. The footage then disappeared.
The lost video will now be part of a 92-minute documentary entitled 'The Beatles: The Lost Concert', which includes an interview with Chuck Berry. It will premiere in New York's Ziegfield Theater on May 6.
The first part of the film will be on the rise of Beatlemania in the United States and contains commentary from Berry, Mark Ronson, Aerosmith pair Steven Tyler and Joe Perry and Albert Hammond Jr and Nick Valensi from The Strokes.
It will then be screened in theatres across America on 17 and 22 May. PTI NDS