The stoppage -- part of rolling industrial action expected to continue until Friday -- was due to hit Frankfurt, Duesseldorf and Munich airports, the UFO flight attendants' union said.
Frankfurt, Germany's main air hub, and Duesseldorf were to be hit by work stoppages from 0330-2200 GMT, and Munich from 0330-2300 GMT, the union said on its website on Sunday.
UFO last Thursday said industrial action had become "unavoidable" after airline management failed to come up with an improved offer in a dispute over pay and early retirement provisions.
The strike yesterday forced Lufthansa to cancel 520 flights, leaving 58,000 passengers grounded.
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The stoppage affected all domestic and European flights from Frankfurt and Duesseldorf, Germany's busiest and third-busiest airports.
Flights resumed for one day Sunday "because on that day, most flights are private", said UFO.
UFO has said it plans to target different airports over the course of the week-long blitz of walkouts.
The union is demanding that a current system of early retirement provisions remain unchanged.
Lufthansa argues that the system is too expensive in the face of competition from low-cost operators such as Ryanair and easyJet.
The pilots, who are also concerned about Lufthansa's aim to further develop its low-cost activities, have staged repeated walkouts during the dispute.
Last week, Lufthansa nonetheless said it was raising its full-year forecasts after low oil prices and positive passenger numbers lifted profits in the third quarter.
Lufthansa shares were among the biggest gainers on the Frankfurt stock exchange on Friday, closing 3.26 per cent up at 13.95 euros (USD 15).