The pop superstar, 57, reportedly started her gig in Louisville three-hours late, and several fans took to social media to accuse the singer of forgetting her lyrics and acting "drunk" during the performance.
"For those people who like to believe all they read I never drink and perform!!! My show is 2 hours and 15 minutes of non stop singing and dancing," she wrote on Instagram.
Madonna goes on to explain that she made a joke during the Louisville show which she fears fans may have misinterpreted.
"Its (sic) so very interesting how society continues to not only treat me in a totally sexist way (if I a were a man no one would have said a thing) and also continue to take everything I say literally! Thats (sic) what happens when people don't read books and get all information from (website) TMZ.