The farmers have launched the agitation to press various demands including a loan waiver.
Curfew was imposed in Yeola in Nashik district where 14 policemen were injured in stone pelting.
Chief Minister Devendra Fadnavis, while expressing willingness to hold talks with the agitating farmers, alleged that it was the opposition NCP and Congress who were instigating them.
A committee has been asked to suggest measures to resolve farmers' issues, he said.
Protesters in Satara, Kolhapur and Nashik districts tried to disrupt supply of milk and vegetables to Mumbai last night.
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Kolhapur District Cooperative Milk Society Ltd, known as Gokul Dairy, filed a police complaint in Satara stating that one of its tankers was attacked on the way to Mumbai.
Police in several districts provided protection to milk tankers heading towards the state capital. Gokul Dairy supplies seven lakh litres of milk per day to Mumbai, which has daily need of 55 lakh litres, said Dattatray Ghanekar, MD of Gokul Dairy.
The average supply of vegetables to Navi Mumbai APMC is around 7,000 tonnes, he said.
Police lathicharged to disperse a mob of some 600 agitators on the busy Yeola-Kopargaon road which was ransacking trucks of vegetables.
Additional Superintendent of Police Dr Rahul Khade, along with two inspectors, a sub-divisional police officer of Manmad and 10 constables were injured as the mob pelted stones, police said.
At Lasalgaon in Nashik district, protesters stoned a police jeep, damaging its windshields. Agitators threw onions, chillies, mangoes and tomatoes on the road as weekly markets in 82 places in the district remained closed.
APMC yards in Nashik wore a deserted look as farmers did not turn up to auction their agriculture produce.
In neighbouring Ahmednagar district, some3,500 milk collection centres and 500 cold houses remained closed.
In Nagpur, Maharashtra's second capital, the agitation didn't have much impact, APMC sources said.
Many farmers emptied milk on the road in Shioor village of Aurangabad district.
Chitale Dairy, milk supplier based in Sangli district, decided not to send its vehicles for milk collection tomorrow as a precautionary measure. It has asked member farmers to send milk to the main collection centre, said Girish Chitale, a partner of the Dairy which supplies milk to Mumbai and Pune.
Police today provided some escort vehicles to give protection to milk collection tankers, he said.
In Nashik, farmers are also opposing the proposed Mumbai-Nagpur 'Samruddhi Corridor' (expressway), which will require acquisition of farmland.