"We will not accept any anti-party activity. If the party is not there, the individual is non-existent," she said addressing a party workers' meeting here in West Midnapur district.
Banerjee's strong reaction is because of some party leaders singing different tunes recently on some issues and reports of group rivalries in some constituencies.
Trinamool Congress MP Sugato Bose recently went on record, saying, "The culprits in the financial fraud (Saradha chit fund scam), whoever they may be, have to be brought to book through the legal process as poor people have been duped."
Bose's comment caused an embarassment to the party at a time when the TMC supremo had launched a scathing attack against the Narendra Modi government and the CBI, terming the arrest of state transport minister Madan Mitra in the Saradah scam as an outcome of "political vendetta".
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She had also directed her MPs to wear black badges and shawls and to protest during the just-concluded winter session of Parliament.
Another leader and state Consumer Affairs Minister Sadhan Pandey had hinted last Saturday without taking name that Jadavpur University vice-chancellor Abhijit Chakrabarti ought to have resigned.
Pandey had said that any person with self-respect should "leave once he realizes that he is unwanted".
These acts have raised eyebrows in the party with TMC spokesperson Partha Chatterjee saying that party functionaries should have expressed their opinions within the party.