An ambulance rammed into a stationary truck on the DND Flyway in the early hours of Friday, killing a man and a two-year-old boy who was being taken to the Safdarjung Hospital, officials said.
Four persons were also injured in the incident which took place near the DND toll tax booth at around 3.15 am, they said.
The deceased have been identified as Sunil Kumar, 30, and Subhash Pal.
Police said Suresh Pal, 35, his wife Manno Pal, 32, and four-year-old child Shubham Pal and ambulance driver Vipin, 25, sustained serious injuries and were first rushed to the Lal Bahadur Shastri Hospital, following which they were referred to the AIIMS Trauma Centre.
The two-year-old boy was sick and was being taken from Noida to the Safdarjung Hospital for treatment when the incident took place, a senior police official said.
According to the ambulance driver and another witness, the truck was stopped by a transport enforcement team after crossing the DND toll.
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After the incident, both the truck driver and his counterparts fled the spot, the official said.
The Delhi Fire Service said they received a call at about 3.40 am after which two fire tenders were rushed for rescue operation.
Ambulance technician Sunil Kumar succumbed to injuries at the Lal Bahadur Shastri hospital while the minor boy was also declared brought dead, the officer added.
"I saw that the driver was in a drowsy condition and I asked to stop the vehicle. He said that he was fine and continued driving," said Suresh Pal, the father of Subhash.
The child's family were also inside the vehicle and suffered minor injuries. They were accompanied by two ambulance technicians, out of which one man has died.
Subhash, who used to live at Knowledge Park in Greater Noida with his family, was admitted at the Sharda Hospital in Noida on Thursday after he complained of breathlessness.
"We were informed that there was some water in Subhash's windpipe and doctors asked us to shift him to Safdarjung Hospital. We left the hospital around 3 am and were heading towards the Safdarjung.
"There was a truck driving in front of us on DND Flyway and we couldn't see anything ahead of it. After the collision, I lost consciousness and could not save my kid," said Mannu Pal, Subhash's mother.
A case has been registered and efforts are on to nab the accused, Deputy Commissioner of Police (East) Jasmeet Singh said.