Dhanna Ram fell on the stage from a height of 10 feet and died on the spot last night, they said.
He was enacting the part from the Ramayana where Hanumana brings 'Sanjeevani Booti'.
Ram was a known local artiste and had been playing Hanumana's role for the last 40 years, police said.
Bikaner MP Arjun Ram Meghwal has expressed his condolences to his family.