Prolonged hammering and chiselling accelerated degenerative arthritis in the hands of Michelangelo, sculptor, painter and one of the greatest artists of all time.
However, the intense work probably helped him keep the use of his hands until he died, researchers said.
Doctors analysed three portraits of Michelangelo between the ages of 60 and 65 which show that the small joints of his left hand were affected by non-inflammatory degenerative changes that can be interpreted as osteoarthritis.
"It is clear from the literature that Michelangelo was afflicted by an illness involving his joints. In the past this has been attributed to gout but our analysis shows this can be dismissed," said lead author Davide Lazzeri, a specialist in plastic reconstructive and aesthetic surgery at the Villa Salaria Clinic in Italy.
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Despite this he continued to create one masterpiece after another and was seen hammering up to six days before his death in 1564, three weeks before his 89th birthday.
By then Michelangelo was unable to write anymore and only signed his letters.
"The diagnosis of osteoarthritis offers one plausible explanation for Michelangelo's loss of dexterity in old age and emphasises his triumph over infirmity as he persisted in his work until his last days," Lazzeri said.
The study was published in the Journal of the Royal Society of Medicine.