Although there are existing techniques to make 3D tissues from heart cells, the new method dramatically reduces the number of cells needed, researchers said.
"We have bio-engineered micro-scale heart tissues with a method that can easily be reproduced, which will enable scientists in stem cell biology and the drug industry to study heart cells in their proper context," said Nathaniel Huebsch, a postdoctoral fellow at the Gladstone Institutes in US.
Creating heart cells from induced pluripotent stem cells (iPSCs) derived from a patient's skin cells enables scientists to study and test drugs on that patient's specific disease.
However, cells made from iPSCs are relatively immature, resembling heart cells in an embryo more than in an adult.
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These cells are inadequate for drug testing because they do not predict how a drug will affect adult heart cells.
Heart cells created from iPSCs are challenging to make and work with, so creating large quantities can be difficult. Therefore, the fewer cells needed, the better.
Also, the new method requires a thousand-fold fewer cells to grow the tissue than other tissue engineering techniques. Using fewer cells allows the scientists to do many more experiments with the same amount of resources.
The researchers first generated heart muscle cells and connective tissue cells from iPSCs. They then combined these cells in a special dish shaped like a tiny dog bone.
For example, when the researchers tested how the tissue responded to certain drugs that impair foetal heart cells but not adult heart cells, the micro heart muscle performed more like adult heart tissue.
"The beauty of this technique is that it is very easy and robust, but it still allows you to create three-dimensional miniature tissues that function like normal tissues," said Bruce Conklin, a senior investigator at Gladstone.
"Our research shows that you can create these complex tissues with a simple template that exploits the inherent properties of these cells to self-organise," said Conklin.
The study was published in the journal Scientific Reports.