The Australian Defence Vessel Ocean Shield is returning to the search area off Western Australia to continue the hunt for missing flight MH370, officials said.
The Beijing-bound plane - carrying 239 people, including five Indians, an Indo-Canadian and 154 Chinese nationals - had mysteriously vanished on March 8 after taking off from Kuala Lumpur.
The ship will deploy underwater vehicle Bluefin-21, which will search the seabed in a location in the southern Indian Ocean where pings from suspected black boxes were detected in April.
The initial deployment of Ocean Shield ran from March 31 until May 5. After five weeks at sea, it returned to port where a software update was run on Bluefin-21's scanner.
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A total of 4.64 million square kilometres of ocean had been searched as of Tuesday, but no sign of wreckage from the plane has been found so far.
Matthews was quoted as saying that the equipment will be used in the same area where sounds consistent with a black box locator were detected last month.
"They'll either find something or they won't, that's about all I can box in, but what you do is you go look at your best indications and you pursue them until they're exhausted," he said.
Matthews said the Bluefin-21 vehicle will be deployed to do a site scan survey to look for "any non-normal items, any metallic items".
"Concurrently there's a team in Canberra that includes Australian Transport Safety Bureau (ATSB), National Transportation Safety Board (NTSB), Boeing and Inmarsat looking at the satellite data, just to take a fresh look, make sure they refine as much as they can the broader search area," he added.
The ship will return at the end of the month, and what happens beyond that will be determined by Malaysian, Australian and Chinese authorities, Mathews said.