Mishra has mentioned seven matters of "corruption" including an "exchange" of Rs two crore between Kejriwal and Health minister Satyendar Jain.
"I have given statement on 7 serious issues of corruption by @ArvindKejriwal and his associates before Hon'ble Lokayukta," he said in a tweet.
The Lokayukta is mandated to enquire into corruption cases at state level.
However, Mishra did not submit any evidence with Lokayukta Reva Khetrapal, saying he would do so later as some of the evidences were with his associate and "independent investigator" Neil Haslam, who was out of town.
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"It is a very serious matter that a public representative has given a statement before the Lokayukta against Kejriwal and now he has no moral right to continue as Chief Minister," BJP's Delhi unit president Manoj Tiwari said.
Mishra was removed as Water Resources and Tourism minster from the Delhi cabinet on May 6 and had made the startling corruption allegation against Kejriwal the next day.
A complaint of "corruption" against Kejriwal and Jain was filed by the Delhi BJP's legal cell following Mishra's allegation before the Lokayukta.
The Lokayukta had sent a notice to Mishra for recording his statement in connection with his allegation.
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