Fallon, who marked his debut as the Golden Globes host, gave a musical start to the evening with a "La La Land" inspired song referring everything from "Star Wars" to Jon Snow's "Game of Thrones" resurrection.
Soon after the video ended Fallon suffered a major technical fail when his auto-cue broke.
The audience, which had some of the biggest names from the Hollywood, could not stop laughing while the host tried his best to make up for the gaffe.
"I can do impressions, I can think of something - cut to Justin Timberlake now. He'll wink at me or something," he added.
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After a minute or so, the second teleprompter was installed, however, Fallon knew he had given the "moment" of the evening.
"Already you have your Golden Globes moment, already. It's already like a GIF," he joked.
The other major blooper was when "Hidden Figures" was mistaken as "Hidden Fences" not once but twice.
"Fences," on the other hand, is a different film starring Denzel Washington and Viola Davis in the leading roles. The film is based on August Wilson's play.
Hager was not the only one to get the movie names wrong. While presenting best supporting actress (in which "Hidden Figures" star Octavia Spencer and "Fences" actress Viola Davis were nominated), Michael Keaton also called the movie "Hidden Fences."
Senior reporter Al Roker accidentally referred to Jessica Biel as the actress Jessica Alba, for which he had to film a new introduction.
Another reporter referred "Lion" child actor Sunny Pawar as Dev Patel's brother. While, Patel was one of the presenters at the award ceremony, he was also nominated for the film in the best supporting actor category, which he lost out to Aaron Taylor-Johnson of "Nocturnal Animals".
Sunny plays the young Saroo Brierley, while Patel is in the grown-up version in the Garth Davis-directed movie about a man searching for his birth mother in India.