Police said no untoward incidents were reported as part of the 12 hour strike called by Tamil Ealam Supporters' Organisation (TESO) to exert more pressure on New Delhi to support a US-sponsored resolution against Sri Lanka at the United Nations Human Rights Council.
DMK chief M Karunanidhi, who had revived TESO last year floated by him in the 1980s, had called for peaceful protests.
DMK workers took to various forms of protests including rail-blockade in various parts of the state and shouted slogans against Sri Lankan President Mahinda Rajapaksa.
Some shops were closed in several parts of the state but life remained largely normal in Chennai and elsewhere with government buses and trains operating smoothly.
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The strike call evoked lukewarm response in the industrial districts of Coimbatore and Tirupur besides in Madurai.
In the hill district of Nilgiris, shops were closed in Gudalur, Kothagiri and Coonoor, affecting normal life, while commercial units remained open at Udhagamandalam,the major tourist spot in the district. Vehicles plied as usual.
The strike call evoked good response in the neighbouring union territory of Puduchery, governed by the AINRC party.
Many DMK activists were taken into custody even as most shops remained closed in response to the strike call.
Superintendent of Police S Ramaraj told PTI two buses belonging to the State Transport Corporation were stoned on the outskirts of Puducherry in the wee hours today.
Meanwhile, students of various colleges in Chennai and some parts of Tamil Nadu continued with their protests seeking independent probe against Colombo for alleged war crimes.