The study, conducted by researchers at the University of Birmingham School of Sport and Exercise Sciences, UK, dispels the myth that coffee consumption can cause dehydration.
Researchers found that drinking moderate amounts of coffee does not result in dehydration and contributes to daily fluid requirements in regular coffee drinkers just as other fluids do.
Due to early research showing the acute effects of caffeine as a mild diuretic, there appears to be a common assumption that caffeinated beverages, such as coffee also have this effect, researchers said.
However, the effect of coffee consumption on fluid balance cannot be directly compared with that of pure caffeine.
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This is the first study to directly assess the effects of a moderate consumption of coffee compared to equal volumes of water.
"Despite a lack of scientific evidence, it is a common belief that coffee consumption can lead to dehydration and should be avoided, or reduced, in order to maintain a healthy fluid balance," said Sophie Killer lead author of the study published in the journal PLOS ONE.
In a sample of regular coffee drinkers, Killer and colleagues measured the effects of moderate consumption of black coffee compared to the consumption of equal volumes of water on fluid balance and hydration status.
Fifty male participants were tested in two phases, where they were required to drink four mugs (200ml) of either black coffee or water per day for three days.
In the second phase, those who had initially drunk coffee switched to water and vice versa. The two phases were separated by a ten day 'wash out' period. Females were excluded from the trial to control against possible fluctuations in fluid balance resulting from menstrual cycles.
Moreover, no differences in 24-hour urine volume or urine concentration were observed between the two groups.
"We found that consumption of a moderate intake of coffee, four cups per day, in regular coffee drinking males, caused no significant differences across a wide range of hydration indicators compared to the consumption of equal amounts of water," said Killer.