The classic comedy troupe, who had announced in November last year that they were getting back together for a series of live shows, have added one more performance to their already sold-out string of shows in London, reported Metro online.
The group confirmed the news of their last show in a joint statement.
"Monty Python, the world's busiest comedians, have reluctantly decided that 20 July 2014 at the O2 London will probably be their last live performance together," they said.
"Don't miss the party, you probably won't get the chance again," they added.