The smashup between an early form of Earth and a planetary body named Theia some 4.5 billion years ago is put forth by what scientists call the Giant Impact Hypothesis of moon formation.
While most experts support the notion, they say the only way to confirm such an impact is to study ratios between the isotopes of oxygen, titanium, silicon and others.
Until now, researchers studying lunar samples that arrived on Earth via meteorites have found that the Earth and moon were quite similar in makeup.
"They were able to detect a slightly but distinctly higher composition of the oxygen isotope in the lunar samples," said the study in the journal Science.
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"This very small difference supports the Giant Impact Hypothesis of moon formation."
According to theoretical models of the collision, the moon would have formed mostly from Theia, at between 70-90 per cent, with some 10-30 per cent coming from Earth.
But researchers now say the moon could be a 50/50 mixture of Earth and Theia remnants, though more study is needed to confirm.
"We can now be reasonably sure that the Giant collision took place."
The findings will be presented at the Goldschmidt geochemistry conference in California on June 11.