Targeting the Mamata Banerjee-led party over the photo episode, Karat said TMC is getting "increasingly desperate" as the poll campaign in West Bengal goes on.
It shows the level to which TMC can "stoop to", he said.
Karat, the former CPI(M) general secretary, has lodged the complaint at Mandir Marg police station in the national capital.
"He (O'Brien) has used morphed picture in his press conference and it has also been put on website of Trinamool Congress and this is being widely shown on the (news) channels also.
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He said displaying the "fabricated" photograph on TMC's website falls under cyber crime and that it was O'Brien who should be held responsible for "using" the image.
Karat said CPI(M) will also take up the issue before people of the state to "expose" the ruling party "politically".
The Trinamool Congress, following a press conference by its national spokesperson O'Brien, had yesterday posted two videos and six pictures on its website. The party, however, removed the morphed picture from its website after controversy.