It called the report from the Syrian Observatory for Human Rights "another falsehood," adding the Russian military targets "only unpopulated areas and international terrorist groups."
"Russian army planes did not hit Al-Shafah in the Deir Ezzor province," it said in a statement.
Yesterday, the Britain-based monitor accused Moscow of killing 34 civilians, among them 15 children, in an air strike on the village Al-Shafah which lies on the eastern bank of the Euphrates River.
The Observatory relies on a network of sources inside Syria, and says it determined whose planes carry out raids according to type, location, flight patterns and munitions used.
More than 340,000 people have been killed in Syria since the conflict began in March 2011 with anti-government protests.