The District Education Officer issued separate suspension orders on Monday, stating the act of reliving in the open amounted to misconduct and the violation of the government's directives.
While one Assistant Teacher is posted at the primary school in Rawsar village another Assistant Teacher at the primary school in Budera village in the district.
DEO Aditya Narayan Mishra stated the wife of the assistant teacher posted at Rawsar has violated Swachh Bharat Mission by not using the toilet at her home.
Mishra said the action was taken after workers of Swachh Bharat Mission found the assistant teacher, a resident of Budera village, defecating in the open and submitted a report to the district collector.
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"In accordance with the directives of the district collector, the education department has suspended both the assistant teachers for open defecation with immediate effect," he added.
Under Swachh Bharat Mission, teachers are entrusted with the task of keeping a watch on people who relive themselves in the open.
In June this year, the panchayat officials in Virpur village in Raisen district had imposed a fine of nearly Rs 4 lakh on 13 families for not using toilets.