In the midst of speculation of his rift with the TMC supremo, Roy, a founder member of the party who has been under CBI scanner in the Saradha chit fund scam, did not figure in the new 21-member working committee set up at today's working committee meeting.
The removal of Roy, who was till recently considered to be the number two in party organisation as the party's all-powerful all-India general secretary came during a fresh organisational shake-up, the second in the past fortnight.
Roy skipped today's meeting presided over by Mamata Banerjee.
Now in Delhi, Roy had told reporters there yesterday that he would not be able to attend today's working committee meeting due to the union budget and some personal work.
Trinmool Congress's official website today said Derek O'Brien, who was functioning as the chief whip of TMC parliamentary party in the Rajya Sabha, has been appointed in Roy's place as leader of the party in the upper House.