Opting to bowl, Mumbai pacers first utilised the seaming conditions at the Sawai Man Singh Stadium pitch before their spinners came to the party to stop Lions from posting a formidable total.
Lions did not have the best of the starts losing both their openers inside the 50-runmark.
Rassie van der Dussen (13) was the first to depart as he mistimed a pull straight to Rishi Dhawan at square leg off Mitchell Johnson.
Coming onto bowl in the seventh over, out-of-favour India off-spinner Harbhajan Singh tasted success with his fourth delivery, trapping Quinton de Kock (19) in front of the wicket.
Veteran Neil McKenzie looked impressive during his 16-run innings. He hit a four and a six before Dhawan went through his defence with a perfect in-swinger.
Mumbai's skipper Rohit Sharma introduced Pragyan Ojha in thhe ninth over and the left-arm spinner produced result immediately by disturbing the timbers of Jean Symes after the batsman clobbered the bowler for a six in the previous delivery.