A grinning Ri Sol-Ju, wearing what appeared to be a wedding ring on her left hand, was pictured clapping next to her smiling husband Kim Jong-Un during a men's football match at Kim Il Sung Stadium yesterday, the North's Korean Central News Agency (KCNA) said.
The images of the couple at a podium flanked by top party officials were published in state media.
The match was being held as part of a lavish series of events celebrating the 103rd birthday of Kim Il-Sung, the young leader's grandfather, which falls on Wednesday.
A former member of the North's Unhasu Orchestra, Ri was last seen in public in December 2014, when she and her husband attended a ceremony to commemorate the third anniversary of the death of Kim Jong-Un's father, Kim Jong-Il.
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Their marriage was only revealed in July 2012 when pictures emerged of a young woman accompanying Kim at official events in a break from the past, when the North's first ladies were kept out of the limelight.
But Ri has been out of the spotlight since December for unknown reasons.
The Kim dynasty has ruled the isolated state for nearly seven decades with an iron fist and pervasive personality cult.
Kim Il-Sung led North Korea from its establishment in 1948 until his death on July 8, 1994.
He was succeeded by his son, Kim Jong-Il, who also ruled until his death in December 2011 when power was transferred to his son and current leader Kim Jong-Un.
Today more than 800 runners took place in an international marathon watched by hundreds of fans, including some 600 from abroad, as part of the celebrations.
"Attending the race were more than 600 marathon fans from dozens of countries including Malaysia, Singapore, Sweden, Finland, UK and Italy," KCNA said.