Jankidevi, Sai's wife of ten years, had earlier given two statements to the police who investigated a case of rape against Sai.
Yesterday, she deposed as a prosecution witness in the court of Additional Sessions Judge P S Gadhvi.
Her relationship with Sai was "not cordial like it should be between husband and wife," and he mostly remained out of (Asaram's) ashram, she said.
In her deposition, she also spoke about activities at the ashram at Motera in Ahmedabad where she lived.
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Next hearing of the case is on May 2.
Sai is currently in Surat's Lajpore jail after he was arrested in the rape case. He is also facing a separate case for hatching a conspiracy to bribe the police and judicial officials to weaken the rape case.
A Surat-based woman has accused Sai of raping her when she was living in Asaram's ashram. The woman's elder sister has also lodged a separate case of rape against Asaram himself.