In a regular two-day meeting of the 28 ministers, including US Secretary of State John Kerry, NATO will confirm the suspension of cooperation with Moscow, a decision made on March 5 after Russian troops grabbed Crimea from Ukraine.
"Reassuring allies is most important for NATO," said Douglas Lute, the US ambassador to the Brussels-based Western alliance, in a pre-meeting briefing.
Hoping to avoid antagonising Moscow, NATO agreed in the 1990s to keep permanent troops out of new member countries that border Russia.
"We cannot do business as usual with Russia," Lute said.
"It is clear that Russia has not played by the rules, has not been consistent with our partnership ... So we can review our own rules."
The US has been at the forefront of responding to Russian provocations in Ukraine, hoping to balance its desire to reinvigorate the NATO alliance while keeping diplomatic channels with the Kremlin open.
Over the weekend, General Philip Breedlove, NATO's supreme allied commander, was sent back to Europe early amid what the Pentagon called Russia's "lack of transparency" over the Ukraine crisis.
Since the Crimea crisis began, NATO has flown AWACS radar aircraft over Poland and Romania, and the US has dispatched fighter jets to Lithuania and Poland.
NATO will hold air drills over the ex-Soviet Baltic countries on Tuesday -- an annual exercise that has gained added significance amid the concerns over Russia's territorial ambitions.
Several other NATO powers, including France and Britain, have also offered additional warplanes, while Germany will allegedly follow suit according to a report from German magazine Der Spiegel.
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