In an interview in Italy with the BBC, Zhanna Nemtsova said that her father was "a critic of Putin, he fought with Putin, with nobody else," and said she blamed the president for his death.
"After his death the opposition is beheaded and everybody is frightened," she said.
The killing of the 55-year-old Nemtsov, who was shot dead near the Kremlin in late February, has infuriated the opposition, which sees Putin as responsible for his death.
Five people have been detained and two of them charged for carrying out the killing, though Nemtsov's colleagues worry that whoever ordered his murder may not be found.