Netmeds founder Pradeep Dadha's family investment fund, boutique investment bank MAPE Advisory Group also participated in the funding, the company said in a statement.
Netmeds.Com is promoted by the Dadha family which has been in the pharmaceutical business for over a century.
"This additional funding will help us put in place a supply chain management system," Pradeep Dadha said.
"We plan to use about USD 10 million of the USD 50 million commitment immediately for back-end development and to augment our logistics infrastructure to service the most under-served cities," he added.
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As part of the financing, OrbiMed's Sunny Sharma will join the company's board of directors.
Netmeds.Com has both prescription drugs and non- prescription (OTC) products, supplying over 50,000 stock keeping units (SKUs) across India from its first operational hub in Chennai.
It also offers the convenience of uploading a prescription and ordering medicine through its mobile app that is available on iOS and Android smartphones.
The company has tied-up with India Post to provide delivery services.
Netmeds.Com had earlier said they are looking at capturing 3-4 per cent of the market share in the pharmacy business by 2020.