The new trail "goes the extra mile with specially curated thematic routes to cater to time-crunched trail-goers. It offers three "bite-sized" routes which cover different characteristics of the neighbourhood, National Heritage Board (NHB) said.
This is NHB's 16th heritage trail.
Two mosques linked to early Indian muslim immigrants as well as Chinese and Buddhist temples are part of the 40 historic sites and 18 markers in the precinct, a tourist thorough fare of street-shops selling spices, fresh produce, flowers of Indian-origins.
The precinct was also known as Village of Lime, or Soonambu Kambam in Tamil, now one of the fourth official language in Singapore, most spoken after English, Chinese and Malay.
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From the late 1820s to about 1860, the British- established lime pits and bricks kilns along Serangoon Road served as a source of employment for many Indians.
Indians immigrants also gravitated there to work in the cattle trade.
When early Indian enclaves such as Chulia Street and High Street in the Central Business District became overcrowded in the early 20th century, many then moved to Serangoon.
"We want them (visitors) to go away from their visit with a betterappreciation of the precinct's rich and multi-cultural heritage," said Tan.
Today, the Little India precinct is popular with Indians for its variety of shops selling consumer products including gold jewelry, as well as foods, hostels and motels.