The House also witnessed acrimonious exchanges between DMK and Congress member Renuka Chowdhury over certain remarks made by her soon after she left the Chair while adjourning the House for the day.
Her remarks infuriated the DMK members who protested strongly leading to a tiff.
Rajya Sabha saw bedlam as two of the four mikes on the Chairman's table were wrenched out and broken by AIADMK members, who were agitating over Sri Lankan Tamils issue.
The trouble did not end even after Chowdhury adjourned the House as she made certain remarks against the protesting members, adding fuel to the fire.
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Infuriated DMK members, including Tiruchi Siva, Selvaganapathi and Vasanthy Stanley strongly protested her remarks, terming them as unparliamentary.
The DMK members also threatened that they will not allow Chowdhury to occupy the Chair in future. "You cannot treat us like animals," they shouted back.
Though Chowdhury left the House, Congress members Ambika Soni, Jairam Ramesh and R P N Singh and some senior members, including those from the opposition, had to intervene to placate the DMK members.
DMK member Siva got so agitated that he required medical aid and an ambulance was called as a standby.
Earlier, while protesting over the Sri Lankan issue, Stanley had fainted in the House on Wednesday and to be rushed to hospital in an ambulance.