Found between 2010 and 2013, the species include a flame-patterned lizard, a thumbnail-sized frog, a vegetarian piranha, a brightly coloured snake, and a beautiful pink orchid, according to World Wildlife Fund (WWF).
Discovered by a group of scientists and compiled by WWF, the new species number 258 plants, 84 fish, 58 amphibians, 22 reptiles, 18 birds and one mammal. The total does not include countless discoveries of insects and other invertebrates.
Some of the most remarkable species outlined in the report include: Flame-patterned lizard, thumbnail-sized frog, vegetarian Piranha, a brightly coloured snake, a beautiful pink orchid and Caqueta titi monkey.
Many of the new discoveries are believed to be endemic to the Amazon rain-forest on the Brazil-Peru border and are found nowhere else in the world.
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This makes them even more vulnerable to rain-forest destruction that occurs every minute across the Amazon, researchers said.
"The destruction of these ecosystems is threatening biodiversity and the services it provides to societies and economies. We cannot allow this natural heritage to be lost forever," Maretti said.