New sports doping test '1,000 times more sensitive'

Researchers have developed a new test to detect performance-enhancing drugs which they claim is 1,000 times more sensitive than current methods.
"How much of a drug someone took or how long ago they took it are beyond the analyst's control. The only thing you can control is how sensitive your method is," said Daniel Armstrong at the University of Texas at Arlington, who led the team.
"Our goal is to develop ultra-sensitive methods that will extend the window of detection, and we have maybe the most sensitive method in the world," Armstrong said.
Hongyue Guo, a graduate student in Armstrong's lab, explained that the new strategy is a simple variation on a common testing technique called mass spectrometry (MS).

The International Olympic Committee, the US Anti-Doping Agency and others routinely use MS to ensure athletes are "clean".
MS separates compounds by mass, or weight, allowing scientists to determine the component parts of a mixture.

In the case of PEDs, technicians use the method to find the bits left over in blood, urine or other body fluids after the body breaks the dopants down.
Because some of the pieces, or metabolites, are small and have a negative charge, they may not produce a signal strong enough for the instrument to detect, Armstrong said, especially in the case of stimulants, which the body rapidly breaks down.

Stimulants like amphetamine, or "speed," increase alertness and reduce an athlete's sense of fatigue.
The method Armstrong's lab has pioneered, called paired ion electrospray ionization (PIESI, pronounced "PIE-zee"), gathers several of those drug bits together, making them more obvious to the detector.
So far, Guo has used PIESI to detect different kinds of steroids and stimulants, as well as alcohol.
Guo has demonstrated that the technique is often sensitive enough to detect one part per billion of the PED metabolite in urine, which he said is up to 1,000 times better than existing methods.
Testing laboratories wouldn't need to purchase new equipment to get PIESI's advantages, according to Guo.

The new method only requires adding one ingredient to existing MS procedures, and Guo noted that the chemical is already commercially available and inexpensive.
"PIESI is going to be useful in so many different areas," Armstrong said.
Already, it is being used to detect compounds crucial to human health such as phospholipids and pesticides and herbicides, as well as other environmental pollutants.
The team described the new approach at a meeting of the American Chemical Society in Dallas.