The green panel also said that a new path to the shrine exclusively for pedestrians and battery-operated cars will be opened from November 24.
The NGT directed that no horses or mules shall be allowed on the new route to the shrine and these anmimals shall be removed slowly from the old path as well.
It also directed the authorities to impose a fine (environment compensation) of Rs 2,000 on anyone found littering the roads as well as the bus stop at the nearby Katra town.
The green panel's directions came during the hearing of a plea filed by an activist seeking directions to stop the use of horses and ponies in Vaishno Devi shrine premises in Jammu, prompting the green panel to seek response from the government on the issue.
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The petitioner had expressed concern over the "pollution and danger to public health" caused by indiscriminate use of horses, ponies, mules and donkeys, to carry pilgrims and goods from Katra to the Vaishno Devi temple.
"The new path which was constructed for Rs 40 crore should be positevely opened to public by November 24. No further time will be granted and, in default, appropriate action will be taken against the concerned authorities.," it said.