Nirmala had revealed her Rio ambitions when she improved her previous best by a huge margin clocking 52.35 secs during yesterday's 400m heats at GMC Balayogi Stadium.
The Haryana quarter-miler today ran the race of her life as she led right from start to finish and recorded a personal best of 51.48 secs, a time that put her on the Rio bus and erased the previous meet mark of 51.73 secs held by M R Poovamma since 2014.
Today's time put Nirmala fourth in the Indian all-time rankings behind Manjeet Kaur (51.04s, 2004), K M Beenamol (51.21s, 2000) and Chitra K Soman (51.30s, 2004).
P T Usha's trainee Jisna Mathew (53.14 secs) and Tamil Nadu's P N Souwndharya (53.85s) took the other medals of the day.
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Reacting on Nirmala's time, Usha said: "the race was really good and it would be great if she maintained her performance in the upcoming meets as well."
Hurdler Jithin Paul from Kerala, who was expected to qualify in men's 400m hurdles today could not make it.
Having clocked a personal best of 49.79 secs earlier this month in Samorin, Slovakia, the QM of 49.40 secs looked well within his reach. However Jithin was a bit slower in the initial stages of the final and clocked only 50.41 secs.
Durgesh Kumar Pal, the inaugural Youth Olympic Games silver medalist, gave a tough fight to Jithin till the last hurdle. However, he was tripped at the final hurdle to fall miserably before the finish.
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In discus throw, Punjab thrower Kirpal Singh Batth obliterated the 16-year-old meet record by hurling the disc to 59.74m. The previous mark was held by Anil Kumar (59.74m) since 2000. It was indeed a big improvement for Kirpal, who had bagged a bronze last year, to win the gold this time.
Defending champion Navjeet Kaur Dhillon retained the gold in women's discus throw with 55.07m.
In a keen contest, the men's high jump field saw three jumpers producing an identical clearance over 2.17m. In a tie-breaker Haryana's Ajay Kumar took the top spot while Ritesh Kumar (UP) and Jagdeep Singh (Punjab) declared the silver and bronze medalists respectively.
Tamil Nadu boys (40.72 secs) and Maharashtra girls (47.26s) clinched the gold medal in 4x100m relay in their respective categories.