"There's no such proposal (for a separate Vidarbha state). Carving out small states is a subject handled by the Centre," Fadnavis said.
The Chief Minister's said this amid noisy protests by the Opposition, within and outside the Lower House, on the contentious issue of formation of a separate Vidarbha state.
BJP's ally Shiv Sena, which is vehemently opposed to any such move, also joined in condemning the proponents of a separate Vidarbha.
Shiv Sena MLA Prakash Abitkar asked Fadnavis to clarify stand on creation of a separate Vidarbha state.
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The Assembly was adjourned for the day after noisy scenes by Opposition legislators over the separate Vidarbha issue, which had disrupted the House proceedings on Friday too.
Shiv Sena MLAs held a protest outside the Assembly building, opposing demand for separate Vidarbha state.
"Maharashtra should remain united," shouted the Sena legislators on the stairs of the main entrance of Vidhan Bhawan.
They displayed a banner depicting BJP MP Nana Patole as "shekhchilli" (a comic character). Patole had last week moved a resolution in the Lok Sabha, seeking formation of a Vidarbha state.
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Earlier in the day, raising the separate Vidarbha issue in the Assembly, Congress-NCP legislators rushed to the well and raised slogans for a united Maharashtra.
Shiv Sena MLA from Kolhapur, Rajesh Kshirsagar, picked up the mace in front of the Speaker but he put it down after the Chief Minister objected.
The Sena legislators also sought an apology from BJP state unit president Raosaheb Danve for backing the demand of a separate Vidarbha state.
"Fadnavis should clarify stand on a separate Vidarbha. The government is trying to cut Maharashtra into pieces," Vikhe Patil told reporters at Vidhan Bhawan.