Officials said the agency issued a provisional attachment order here attaching the assets of Ashok Jain and Mahaveer Hirani under the provisions of the Prevention of Money Laundering Act (PMLA).
"The investigations revealed that Rs 10.25 crore of cash and 6.5 kg gold (valued at Rs 1.80 crore) seized by the Income Tax authorities from the premises of Jain and others are assets involved in the process of money laundering and are liable to attachment pending confiscation.
Both Jain and Hirani had been arrested by the ED in this case in December last year.
A total of five people have been arrested till now in this case including Reddy by the central probe agency.
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The agency had filed a criminal complaint against Reddy and others based on a CBI FIR in the case which was registered after the I-T department first searched his and his associates' premises in November last year.
The tax department has made one of the biggest detection of alleged unaccounted income of over Rs 142 crore in this case with the seizure of Rs 34 crore in new notes, post demonetisation.
An attachment of assets action under PMLA is aimed to deprive the accused from obtaining benefits of their ill- gotten wealth and it gets confirmed after an order of the Adjudicating Authority of the said Act within 180 days.