Filing of nominations will now start for 234 constituencies in Tamil Nadu, 140 constituencies in Kerala and 30 seats in Puducherry.
The last date to submit nomination papers is April 29 and it would be scrutinized the next day. May 2 will be the last day for withdrawal. Counting of votes is on May 19.
Around 5.6 crore people are eligible to cast their franchise in Tamil Nadu where about 65,000 polling booths will be set up.
Among the top leaders who campaigned were AIADMK supremo and Chief Minister J Jayalalithaa, DMK President M Karunanidhi, his son M K Stalin, DMDK founder Vijayakant, MDMK's Vaiko and PMK's Chief Ministerial candidate Anbumani Ramadoss.
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(REOPENS DEL21)
In Kerala, the total strength of voters is 2,56,27,620 and 1,33,01,435 of them are women.
Soon after the notification was issued, several candidates filed their nominations.
The filing of nominations began at 11 am at district collectorates.
Former Kerala Finance Minister K M Mani, Forest Minister Thiruvanchoor Radhakrishnan, BJP state President Kummanam Rajasekharan and party's former state unit chief V Muraleedharan were among those who filed their nominations on the first day.
Mani, who holds the record of longest serving member of the state Assembly, had to resign from the Oommen Chandy Cabinet in the wake of the corruption allegations against him in bar bribery scam.